Preliminary work towards homing CRISPR gene drives in Cochliomyia hominivorax - North Carolina State University

Cochliomyia hominivorax

New world screwworm

Intended use

Reduce impacts on livestock in South America and Caribbean caused by this species

Readiness of Technology

3 - Preliminary laboratory work published

Project leader

Scott MJ

Institution

North Carolina State University, USA

Publications

Scott et al., 2020

Paulo et al., 2019

Novas et al., 2023

Authors name for system

autonomous CRISPR/ Cas9-based homing gene drive

Class of Technology

Homing gene drive

Field(s) of application

Agriculture

Description of system

Homing CRISPR

For specialists

Description

Propose a gene drive targeting genes required for female development (Chdsx) or female fertility (Chnudel) (Scott et al. 2020). Chtra also proposed as target (Paulo et al. 2019).

Notes on readiness of technology

Genome sequencing identified target genes, Chdsx and Chnudel (Scott et al. 2020).

+ Protocols established for using CRISPR to modify genome (Paulo et al. 2019).

Phenotype

NA – preliminary study only

Authors name for construct(s)

NA – preliminary study only

Research in target organism

✖

Research in model organism

✖

Intended for release

✖

Intended direct effects

Population suppression/eradication