‘X-shredder’ (I-Ppo-I based) in Anopheles arabiensis - Imperial College

Intended use

Reduction of transmission of malaria pathogen by this vector (to reduce morbidity and mortality from malaria in Sub-Saharan Africa)

Readiness of Technology

6 - Laboratory proof of concept

Project leader

Crisanti A

Institution

Imperial College London, UK

Publications

Authors name for system

Synthetic sex ratio distortion system

Class of Technology

Sex ratio distorter

Field(s) of application

Vector control

Description of system

Autosomal sex distorter -‘X-shredder’

Description

I-PpoI nuclease based autosomal ‘X-shredder’ sex ratio distorter

(Mendelian inheritance)

Notes on readiness of technology

Assumed to be validated in laboratory given that an equivalent A. gambiae strain progressed to large cage trials.

Phenotype

>95% male offspring

I-PpoI expressed as GFP fusion protein

Authors name for construct(s)

gfp124L-2 construct - now renamed to PMB1 (Paternal Male Bias) - introgressed from Anopheles gambiae strain

Research in target organism

✖

Research in model organism

✖

Intended for release

✖

Intended direct effects

Population suppression/eradication