Homing CRISPR in Anopheles stephensi - UC San Diego

Asian malaria mosquito

Intended use

Reduction of transmission of malaria pathogen by this vector (to reduce morbidity and mortality from malaria in India and surrounding regions)

Readiness of Technology

5 - Limited proof of concept in laboratory

Project leader

Bier E

Institution

University of California San Diego

Project leader

James A

Institution

University of California Irvine

Publications

Gantz et al., 2015

Pham, et al., 2019

Authors name for system

Cas9 mediated gene drive system for population modification

Class of Technology

Homing gene drive

Field(s) of application

Vector control

Description of system

Homing CRISPR

For specialists

Description

Homing CRISPR gene drive targeting kynurenine hydroxylase white gene carrying anti-malarial antibody cassette (m2A10-m1C3).

(subject to emergence of resistance)

Notes on readiness of technology

Limited proof of principle – authors note emergence of resistance alleles due to NHEJ and extinction of cage populations due to loss of kh activity in females.

Phenotype

Resistance to malaria parasite, P. falciparum + Red eye marker

Authors name for construct(s)

AsMCRkh2

Research in target organism

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Research in model organism

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Intended for release

✖

Intended direct effects

Population modification/replacement