Preliminary work towards a homing CRISPR gene drive in Vespula vulgaris - University of Otago & Victoria University of Wellington

Common wasp

Intended use

Reduction of ecological effects of invasive populations of this species in New Zealand and elsewhere (e.g. Australia)

Readiness of Technology

2a - Gene drive proposed with supporting theoretical work

Project leader

Dearden PK

Institution

University of Otago, New Zealand

Project leader

Lester PJ

Institution

Victoria University of Wellington, NZ

Publications

Authors name for system

NA – preliminary theoretical study

Class of Technology

Homing gene drive

Field(s) of application

Agriculture

Conservation

Description of system

Homing CRISPR

Description

Homing CRISPR targeting a spermatogenesis gene. Possible target genes include: boule1, ocnus, and sdic genes.

Notes on readiness of technology

Modelling study suggesting feasibility of targeting this haplodiploid species with a suppression gene drive targeting male fertility.
Tested CRISPR- Cas cleavage of possible target genes in vitro.
Genome sequencing undertaken.

Phenotype

Partially infertile males

Research in target organism

✖

Research in model organism

✖

Intended for release

✖

Intended direct effects

Population suppression/eradication